PortaCellTec biosciences GmbH

Drug Transporter Assays

PortaCellTec Biosciences offers a wide range of transporter assays. All transporters are stably expressed in HEK293 cells (human embryonic kidney cells) and each transporter is validated with the following probe substrates and inhibitors.

 

Transporter Gene Probe substrates Probe inhibitors
MDR1* ABCB1 rhodamin123 cyclosporineA, verapamil, LY335979
BCRP* ABCG2 estrone-3-sulfate Ko143, elacridar, sulfosalazine
BSEP* ABCB11 taurocholate cyclosporineA, glibenclamide, rifampicin
MRP2 ABCC2 5(6)-carboxy-
2,'7'-dichlorofluorescein (CDCF)
MK-571
benzbromarone
OCT1* SLC22A1 1-methyl-4-phenylpyridinium decynium22, quinine, quinidine
OCT2* SLC22A2 1-methyl-4-phenylpyridinium decynium22, quinine, quinidine
OCT3 SLC22A3 1-methyl-4-phenylpyridinium decynium22, quinine, quinidine
OCTN2 SLC22A5 L-carnitin verapamil, quinidine
OAT1* SLC22A6 para-aminohippuric acid probenecid, glibenclamide
OAT2 SLC22A7 cyclic GMP indomethacin, bumetanide
OAT3* SLC22A8 estrone-3-sulfate probenecid, indomethacin
OAT4 SLC22A11 estrone-3-sulfate probenecid, sulfobromophthalein
MATE1 SLC47A1 1-methyl-4-phenylpyridinium, metformin cimetidine, quinidine,
ketoconazol
MATE2-K SLC47A2 1-methyl-4-phenylpyridinium, metformin cimetidine, quinidine,
ketoconazol
OATP1A2 SLCO1A2 estrone-3-sulfate verapamil, sulfobromophthalein
OATP2A1 SLCO2A1 prostaglandin E2 diclofenac
OATP1B1* SLCO1B1 estrone-3-sulfate rifampicin, cyclosporinA,
ketoconazol
OATP1B3* SLCO1B3 sulfobromophthalein,
cholecystokinin-8 (CCK-8)
rifampicin, cyclosporinA,
ketoconazol
OATP2B1 SLCO2B1 estrone-3-sulfate rifampicin, cyclosporin
PEPT1 SLC15A1 glycylsarcosine losartan
PEPT2 SLC15A2 glycylsarcosine losartan, Thy-Phe
NTCP SLC10A1 estrone-3-sulfate cyclosporineA
ASBT SLC10A2 taurocholate sulfobromophthalein, fluvastatin
SOAT1 SLC10A6 estrone-3-sulfate sulfobromophthalein
NADC-3 SLC13A3 succinate dimethylsuccinate
oat1 (rat) Slc22a6 para-aminohippuric acid probenecid, glibenclamide
oat3 (rat) Slc22a8 estrone-3-sulfate probenecid, indomethacin
oct1 (rat) lc22a1 1-methyl-4-phenylpyridinium decynium22, quinine


*recommended by FDA/EMA

Under R&D:

Uptake transporters: URAT1, GLUT9 (short and long), OCTN1
Efflux transporters: MRP1, MRP4, MDR3




 

Service


Drug transporter assays

  Study design

  Assay performance

  Inhibition studies

  Substrate studies


Additional service

Partnered service

PortaCellTec Biosciences GmbH

 

Weender Landstraße 94 -108
37075 Göttingen
Tel : +49 551 309 66440
E-mail: PortaCellTec




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News

New validated products:

  • mNtcp
  • mOct1

Events

3rd German Pharm-Tox Summit
Göttingen, Germany
26 February - 1 March 2018


Pharmaforum
Saarbrücken, Germany
8 March 2018

 

AAPS Workshop
Virginia, USA
16 - 18 April 2018

 

20th Barrier- and
Transporter-Meeting

Bad Herrenalb, Germany
7 - 9 May 2018

Greifswalder Transporttage 2018
Greifswald, Germany
7 - 9 September 2018

 


Guidance for Industry (FDA and EMA)

Publications