PortaCellTec biosciences GmbH


Molecular structure and Tissue distribution
Recently, we have cloned human sodium-dependent organic anion transporter (SOAT) cDNA, which consists of 1502 bp and encodes a 377-amino acid protein. SOAT shows 42% sequence identity to the ileal apical sodium-dependent bile acid transporter ASBT and 33% sequence identity to the hepatic Na+/taurocholate-cotransporting polypeptide NTCP. The human SOAT gene is located on chromosome 4q21. SOAT protein exhibits a seven-transmembrane domain topology with an outside-to-inside orientation of the N-terminal and C-terminal ends. SOAT mRNA is most highly expressed in testis. Relatively high SOAT expression was also detected in placenta and pancreas.

Interaction of SOAT with endogenous compounds and drugs
SOAT-HEK293 cell line showed sodium-dependent transportSoat of dehydroepiandrosterone sulfate, estrone-3-sulfate, and pregnenolone sulfate with apparent Km values of 28.7, 12.0, and 11.3 μm, respectively. Although bile acids, such as taurocholic acid, cholic acid, and chenodeoxycholic acid, were not substrates of SOAT, the sulfoconjugated bile acid taurolithocholic acid-3-sulfate was transported by SOAT-HEK293 cells in a sodium-dependent manner and showed competitive inhibition of SOAT transport with an apparent Ki value of 0.24 μm. Several nonsteroidal organosulfates also strongly inhibited SOAT, including 1-(ω-sulfooxyethyl)pyrene, bromosulfophthalein, 2- and 4-sulfooxymethylpyrene, and α-naphthylsulfate. Among these inhibitors, 2- and 4-sulfooxymethylpyrene were competitive inhibitors of SOAT, with apparent Ki values of 4.3 and 5.5 μm, respectively, and they were also transported by SOAT-HEK293 cells.



New validated products:

  • MRP2
  • roct1


2nd German Pharm-Tox Summit
Heidelberg, Germany
March 06-09, 2017

19th Barrier- and

Bad Herrenalb, Germany
May 15-17, 2017

14th European ISSX Meeting
Köln, Germany

June 26- 29, 2017

2nd Symposium on Transporters in Drug Discovery
London, UK
May 15-16, 2017

Münster Transporttage 2017
Münster, Germany
October 21 – 22, 2017


Guidance for Industry (FDA and EMA)